Colony-direct Plasmid Amplification Kit

Product number:CD3224

Product Description: This kit uses DNA polymerase with extremely high fidelity and optimized reaction system to quickly amplify plasmids from bacteria or colonies to obtain DNA that can be used for sequencing reactions. The use of this kit for the preparation of plasmid DNA for sequencing reactions can save a lot of time and cost of shaking bacteria, simplify the sample preparation process, and speed up the speed of receiving samples to the output of results. Each reaction normally produces about 2 μg of plasmid DNA, which can be used directly in the sequencing reaction.

Product composition:

Method of use:

1. Shake and mix each component before use, and place 5 μL sample Buffer v3 in PCR tube;

2. Take the colony/liquid sample to be amplified:

Bacterial solution: directly take 0.2-0.5μL of bacterial solution and add it into a PCR tube containing 5μL sample Buffer v3;

Colony: pick a small number of colonies into a 5μL sample Buffer v3 PCR tube (avoid picking the medium, just touch the colony);

3, short-term centrifugal collection, 95 ℃ treatment for more than 3min for denaturation;

4. After denaturation, the samples were immediately placed on ice for cooling;

5. Preparation of reaction mixture:

6. adding the reaction solution to the PCR tube containing the denatured sample and mixing;

7. After incubating at 30 ℃ for 3 hours, incubate at 65 ℃ for 5 minutes;

8. Take 1μL of amplification product for sequencing reaction (the reaction system can be appropriately adjusted according to needs)

Save condition:-30~-15 ℃ storage, ≤ 0 ℃ transportation

Scope of application:Suitable for bacterial liquid or colony samples, used to prepare plasmid samples required for sequencing.